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FCV Feline Calici Virus PCR Kit Real Time Feline Cat Test Kit Quantitative

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FCV Feline Calici Virus PCR Kit Real Time Feline Cat Test Kit Quantitative

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Brand Name : Biokey

Model Number : Bik-Q-P001

Certification : ISO 13485

Place of Origin : Guangzhou, China

MOQ : we produce both liquid and lyophilized kit

Price : USD

Payment Terms : T/T, L/C, Western Union

Delivery Time : Depending order quantity

Packaging Details : carton package

Parameter : FCV

Specimen : Feline/Cat Eye, Mouth And Nose Secretions

Packing : 8T/box

LoD : 30copies/μL

CV : ≤5%

Applicable : Micgene 242

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Feline Calici virus (FCV) PCR kits, real time quantiative PCR Fluorescent Probe Kits

Intended use:

This kit is used for the specific detection of feline calicivirus (FCV) in cat eye, nose and mouth secretions.

Principle:

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat goblet virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of cat calicivirus was detected by fluorescence PCR in a completely closed reaction system.

Biokey Pet PCR testing solution.pdf

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube

Storage: -20±5℃

After sample preparation, proceeding to PCR thermal cycling below steps:

step cycle termperature time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s

Details please check IFU!


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