Multiple Feline Cat Digestive GI Pathogens Nucleic Acid detection kit PCR Taqman Probe
Intend for Use:
This kit is suitable for the specific detection of feline parvovirus (FPV), feline coronavirus (FCoV), fetal trichomonas (TF) and giardia (GD) in cat eye, mouth and nose secretions and other samples.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose cat tiny virus, coronavirus, SAN MAO trichomonas fetus, jia drops of giardia conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, and the nucleic acids of feline parvovirus, feline coronavirus, fetal trichomonas and giardia were detected by fluorescence PCR instrument in the completely closed reaction system.
Component:
| Composition | Quantity |
| FPV/FCoV PCR liquid | 8 tube |
| TF/GD PCR liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1tube |
Sample requirements:
1. Sample type
Cat eye, mouth and nose secretion and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 94℃ | 2min30s |
| 3 | 45 | 94℃ | 10s |
| 60℃ | 20s collect fluorescent |
FAM and VIC/HEX were selected for detection channel.
Positive judgment value or reference interval:
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 38＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product picture:
Product Tags:
|
|
Multiple Feline Digestive Test Kit PCR Taqman GI Test Nucleic Acid Detection Images
|
